PepWisePepWise
Start free
// Free tool

Reconstitution calculator.
mg, mL, units.

Enter your vial amount, the bacteriostatic water you add, and your target dose. Get the concentration, the exact volume, and the units to draw on a U-100 insulin syringe. The same math PepWise runs for your whole stack.

FREE · NO SIGNUP · RUNS IN YOUR BROWSER

9:415G  ▮▮▮
PepWise
Reconstitution
5 mg vial · 2 mL BAC · 250 mcg
DRAW TO
10u
2.50
MG/ML
0.10
ML

How peptide reconstitution works

Tip: vials are physical glass vials, usually 3 mL or 5 mL. You can’t add more bacteriostatic water than the vial holds, so your water amount is capped by the vial size.

Reconstitution means mixing a lyophilized (freeze-dried) peptide with bacteriostatic water so it can be drawn into a syringe. The math is simple: concentration (mg/mL) = vial amount (mg) ÷ water added (mL). To find how much to inject, divide your desired dose by the concentration to get the volume, then multiply by 100 to get insulin-syringe units.

Worked example

A 5 mg vial with 2 mL of water is 2.5 mg/mL, or 2500 mcg/mL. For a 250 mcg dose: 250 ÷ 2500 = 0.1 mL = 10 units on a U-100 syringe.

Track it automatically with PepWise

PepWise does this math for every peptide in your stack and logs each dose, injection site, and how you feel over time. It is free to start on iPhone, Android, and web.

FAQ

How do you calculate peptide reconstitution?

Concentration equals the vial amount in milligrams divided by the bacteriostatic water added in milliliters. Dose volume equals your desired dose divided by that concentration. On a U-100 insulin syringe, units equal the dose volume in milliliters times 100.

How much bacteriostatic water should I add to a peptide vial?

There is no single correct amount. More water makes each unit easier to measure but dilutes the dose. Many people add 1 to 3 mL to a vial and let the math set the dose volume. Choose an amount that makes your target dose easy to draw.

What is a U-100 insulin syringe unit in mL?

On a U-100 insulin syringe, 100 units equals 1 mL, so 1 unit equals 0.01 mL.

PepWise is an informational and educational tracking tool, not medical advice and not a medical device. Peptides may be experimental or restricted; consult a licensed healthcare provider before starting, changing, or stopping any protocol. Operated by STC Consulting, Inc.